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| Cell line: | EHEB |
| DSMZ no.: | ACC 67 |
| Species: | human (Homo sapiens) |
| Cell type: | chronic B cell leukemia |
| Origin: | established from the peripheral blood of a 69-year-old woman with B-CLL (chronic lymphocytic leukemia) prior treatment by EBV-transformation in 1988; cell line may represent rather a B-lymphoblastoid cell line than a bona fide B-CLL cell line |
| Reference(s): | 14916 |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | lymphoblastoid, round to polygonal, single or clustered cells in suspension; image; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml; cells may grow slowly initially; use at first 24-well plates and 20% FBS; during growth phase split at ca. 1:2 every 2-3 days and maintain at 0.1-0.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-70 hours |
| Harvest: | maximal density at about 1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3-, CD10-, CD13-, CD19+, CD20+, CD34-, CD37+, CD38+, CD80+, CD138(+), HLA-DR+, sIgG+, cIgG+, sIgM-, cIgM-, sIg-kappa+, cIg-kappa+, sIg-lambda-, cIg-lambda-, cyCD79a+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 1.6% polyploidy - 45(42-46)<2n>X, -X - no structural rearrangements detected |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
