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Cell line: | SCLC-21H |
DSMZ no.: | ACC 372 |
Species: | human (Homo sapiens) |
Cell type: | small cell lung carcinoma |
Origin: | established from the pleural effusion of a 46-year-old man with small cell lung carcinoma; patient had received chemotherapy; sister cell line of cell line SCLC-22H (DSMZ ACC 373); listed in the CCLE (ref 18232) |
Reference(s): | 14381, 14382, 18232 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | growing in suspension in small or big clusters (sheets), but do not adhere to the plastic surface; difficult to disperse;
image; image; image |
Medium: | 90% DMEM (4.5 g/L glucose) + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca. 0.3 - 1.0 x 106 cells/ml; split 1:2 every 2-3 days; cells grow in clumps and are difficult to disperse; viability may decrease after thawing and when dispersed too vigorously; cell counting may be difficult, better after digestion with accutase (sedimented cells, 3-5 ml accutase, 10 min) |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 2 days |
Harvest: | cell harvest of ca. 1 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypodiploid karyotype with 12% polyploidy - 42(40-42)<2n>XY, +1, -2, -5, +6, -10, +11, -17, -18, -19, -21, +2mar, del(1)(p22p32), add(5)(p15), del(6)(q14), add(8)(p21), add(11)(q24), del(11)(p11), t(12;17)(q10;q10), del(13)(q12q14), add(15)(q25) - resembles published karyotype (and that of SCLC-16HC) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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