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Cell line: | FL-106 |
DSMZ no.: | ACC 620 |
Species: | rat (Rattus norvegicus) |
Cell type: | cholangio-hepatocellular tumor |
Origin: | established ca. 1986 from the liver of a diethylnitrosamine-treated male Wistar AF/Han rat |
Reference(s): | 15145 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epitheloid cells growing adherently in monolayers |
Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/80 cm2; split confluent culture 1:2 to 1:4 every 2-4 days using trypsin/EDTA for ca. 10 min. |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 2-3 days |
Harvest: | cell harvest of ca. 5 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
Viruses: | PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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