| | |
|---|
| Cell line: | JVE-103 |
| DSMZ no.: | ACC 807 |
| Species: | human (Homo sapiens) |
| Cell type: | colon carcinoma |
| Origin: | established from a liver metastasis of a 51-year-old man with colorectal adenocarcinoma (Duke stage D); cells were described in the literature to exhibit a homozygous deletion of the SMAD3 gene |
| Reference(s): | 18128, 18149 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently in mono- and multilayered clusters; however, cells do not grow to complete confluency; image; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split culture 1:2 to 1:5 every two weeks using trypsin/EDTA (we recommend to wash the cells at least two times with PBS and incubate with trypsin for a minimum of 5 min.); the cells need 3-5 days for recovery and adherence after both thawing and trypsinization; cell number may be difficult to determine due to aggregate formation |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 10-14 days |
| Harvest: | cell harvest of ca. 10 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
