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| Cell line: | KMOE-2 |
| DSMZ no.: | ACC 37 |
| Species: | human (Homo sapiens) |
| Cell type: | acute erythremia |
| Origin: | established from the peripheral blood of a 2-year-old girl with acute erythremia (Di Gugliemo's disease, erythroblastosis) at diagnosis in 1978; cells were described in the literature to produce hemoglobin |
| Reference(s): | 14823 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, large, round to oval cells in suspension; image; image; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.3-0.4 x 106 cells/ml; maintain at 0.2-0.3 x 106 cells/ml; note that after thawing cell viability may be as low as 50%, but cells will recover quickly within a few days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | doubling of ca. 40-50 hours |
| Harvest: | maximal density of 0.6 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD13 +, CD15 -, CD19 -, CD33 +, CD34 -, CD71 +, CD235a -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypotetraploid karyotype (mode weak) with 6% polyploidy - 82(80-88)<4n>XX, -X, -X, -10, -13, -14, -16, -18, -20, -22, +3mar, der(1)t(1;?)(q21;?)x2, del(7)(p15)x2, del(9)(p12), i(17q) - karyotype appears to represent derivative of that originally reported |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
