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Cell line: | KP-363T |
DSMZ no.: | ACC 808 |
Species: | human (Homo sapiens) |
Cell type: | colon carcinoma |
Origin: | established from the tumor mass of an 80-year-old man with colorectal adenocarcinoma (Dukes stage B2) from the left side; described in the literature to carry mutations in the BRAF, PIK3CA, and TP53 genes, a deletion in the SMAD4 gene, and hypermethylation of the CDKN2A promoter |
Reference(s): | 18128 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | epithelioid cells growing adherently as monolayer and in densely packed islets; image; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split confluent culture 1:5 once a week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50 hours |
Harvest: | cell harvest of up to 80 x 106 cells/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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