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Cell line: | X63AG8.653 |
DSMZ no.: | ACC 43 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | myeloma |
Origin: | X63AG8.653 is a non-secreting clone derived from P3X63AG8 (a Balb/c myeloma) and is recommended as fusion parent for production of hybridomas |
Reference(s): | 14655, 14681 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single, round, large cells in suspension; image; image; image; image; image; image |
Medium: | RPMI 1640 + 10-20% h.i. FBS |
Subculture: | seed out at about 1 x 106 cells/ml; maintain at 0.1-1.0 x 106 cells/ml; split culture in the logarithmic phase 1:10 to 1:20 every 48 hours; cultivate initially with 20% FBS |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 30-50 hours |
Harvest: | maximal density of ca. 1.5-2.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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