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Cell line: | KPL-1 (MCF-7) |
DSMZ no.: | ACC 317 |
Species: | human (Homo sapiens) |
Cell type: | breast carcinoma |
Origin: | this cell line is a subclone of MCF-7 (DSMZ ACC 115) with comparable but not identical properties; differences in expression of MUC-1 and the androgen receptor were described
; RNA sequence data are available (see Ref 40906 and RNA-Seq) |
Reference(s): | 14975, 15191, 15219, 18201, 18232, 18234, 40236, 40906 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent growing in monolayers (very dense colonies); image; image; image |
Medium: | 90% DMEM + 10% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2; split confluent culture 1:4 to 1:8 once or twice a week using trypsin/EDTA |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 50 hours |
Harvest: | cell harvest of ca. 5 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database, identical to that of cell line MCF-7 |
Cytogenetics: | human highly rearranged hypertriploid karyotype with 4% polyploidy - 77(70-82)<3n>XX, -X, -1, +2, -11, -13, +14, +15, -17, -18, -19, -20, -20, -21, -22, +15mar - add(X)(q28), add(2)(q35), add(3)(p25)x2, add(5)(p15), add(7)(p14), add(7)(p14), add(8)(p12), add(9)(p22), add(10)(p13)x2, add(19)(q13) - matches published karyotype |
Molec. Genetics: | Western blot: ER and AR positive; PR and HER2 negative (different to MCF-7) |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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