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| Cell line: | L-5178-Y |
| DSMZ no.: | ACC 320 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | T cell lymphoma |
| Origin: | established from an 8-month-old female DBA/2 mouse with T cell lymphoma in 1985 |
| Reference(s): | 14522, 14523 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | grows as single or aggregated round cells in suspension; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at 0.1-0.5 x 106 cells/ml; split saturated culture 1:2 to 1:5 every 1-2 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 20 hours |
| Harvest: | max. density at about 2.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine near diploid karyotype with 13% polyploidy - 39(37-42)<2n> - 1 centric fusion marker |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
