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| Cell line: | MFE-319 |
| DSMZ no.: | ACC 423 |
| Species: | human (Homo sapiens) |
| Cell type: | endometrium adenocarcinoma |
| Origin: | established from the primary adenosquamous endometrium carcinoma (grading G1-G2) of an 81-year-old woman in 1992; described in the literature to form poorly differentiated tumors in nude mice |
| Reference(s): | 14587 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like adherent cells growing as monolayer; image; image |
| Medium: | 40% RPMI 1640 + 40% MEM (with Earle's salts) + 20% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/25 cm2; split confluent culture 1:2 to 1:3 every 5 days using trypsin/EDTA (cells are difficult to detach from the flask; thoroughly washing with PBS and incubation with trypsin/EDTA of 15 min or longer at 37 °C is recommended) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 70-90 hours |
| Harvest: | cell harvest of ca. 15-20 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | originally negative in DAPI, microbiological culture, RNA hybridization, and continuously by PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 4% polyploidy - 45(43-46)<2n>X, -X, t(13;18)(q14.3-21;q11-12) - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
