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| Cell line: | 3T6 |
| DSMZ no.: | ACC 202 |
| Species: | murine (mouse, Swiss albino mouse) (Mus musculus) |
| Cell type: | fibroblasts |
| Origin: | established from disaggregated Swiss albino mouse embryos in 1963; cells were described in the literature to secrete collagen and hyaluronic acid |
| Reference(s): | 15015, 15016 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | fibroblasts growing adherently as monolayer; image; image; image |
| Medium: | 85-90% DMEM + 10-15% h.i. FBS |
| Subculture: | split confluent culture ca. 1:10 every 2-3 days using trypsin/EDTA, seed out about 2 x 106 cells/175 cm2; cells may clump after trypsinization and may be difficult to disperse |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 15 hours |
| Harvest: | cell harvest of about 15 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine flat-moded hypertriploid/hypotetraploid karyotype - 67(64-73)<3n> |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
