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Cell line: | SK-BR-3 |
DSMZ no.: | ACC 736 |
Species: | human (Homo sapiens) |
Cell type: | breast carcinoma |
Origin: | established from the pleural effusion of a 43-year-old woman with invasive breast adenocarcinoma in 1970 after radiation and chemotherapy. Widely used and extensively characterized cell line; decribed as ER-negative, but HER2neu positive; listed in the CCLE (ref 18232); RNA sequence data are available (see Ref 40906 and RNA-Seq) |
Reference(s): | 17266, 18232, 18258, 18234, 18235, 40906 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | adherent cells growing in monolayers changing from round shape after seeding to epithelial-like cells later on; image; image; image; image; image |
Medium: | 80% McCoy´s 5a + 20% h.i. FBS |
Subculture: | seed out at ca. 1-2 x 106 cells/80 cm2 flask; split semi-confluent culture 1:3 to 1:5 once or twice a week using trypsin/EDTA for 5-10 min |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 2-3 days |
Harvest: | cell harvest of ca. 5 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Molec. Genetics: | Western blot: ER, PR and AR negative but HER2 positive |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV -, BPyV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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