| | |
|---|
| Cell line: | MFM-223 |
| DSMZ no.: | ACC 422 |
| Species: | human (Homo sapiens) |
| Cell type: | breast carcinoma |
| Origin: | established from the pleural effusion of a post-menopausal patient with untreated breast carcinoma (tumor: widespread ductal mammary carcinoma T4 N2 M1 with histological grade III); cells were described in the literature to express androgen, estrogen and progesterone receptors (different to own data of DSMZ); RNA sequence data are available (see Ref 40906 and RNA-Seq) |
| Reference(s): | 14585, 40906 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent cells growing as monolayer with reticulate growth pattern; image; image; image |
| Medium: | 85% MEM (with Earle's salts) + 15% h.i. FBS + 2 mM L-glutamine + 1x insulin-transferrin-sodium selenite |
| Subculture: | seed out at ca. 1 x 106 cells/25 cm2; cells grow very slowly; split confluent culture 1:2 every 3-4 days using trypsin/EDTA |
| Incubation: | at 37 °C with CO2 |
| Doubling time: | ca. 5-7 days |
| Harvest: | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human highly rearranged near-diploid karyotype with 12% polyploidy - 46(45-46)<2n>X, -X, -2, -5, -6, +7, -9, -10, +11, +12, -14, -15, -16, -17, -18, -19, -22, +10mar, add(5)(p15), der(7)?inv(7)(q1?q2?)dup(7)(q31q35), add(7)(q11;hsr), der(7)add(7)(p22)add(7)(q31-32), add(8)(p22-23), add(8)(q?24), del(10)(q2?), add(11)(q22), dup(12)(q13q14), add(13)(p11), add(15)(p11), add(17)(p1?), add)19)(q13), add(21)(p11), add(?22)(q13) - carries multiple hsr including ch 7 material - resembles published karyotype |
| Molec. Genetics: | triple-negative due to absence of ER, PR, and HER2 (while positive for AR) in Western blots |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
