| | |
|---|
| Cell line: | GH3 |
| DSMZ no.: | ACC 469 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | pituitary tumor |
| Origin: | derived in 1965 from a pituitary tumor induced in a female Wistar/Furth rat following repeated transplantation with the MtT/W5 tumor (originally induced in a female F/Fu rat by cranial X-irradiation) |
| Reference(s): | 15004, 15008 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing adherently in loosely adherent in semi-confluent monolayers and as cell clusters in suspension; image; image; image |
| Medium: | 82.5% Ham's F10 + 15% horse serum + 2.5% h.i. FBS + 2 mM L-glutamine |
| Subculture: | split confluent culture 1:2 to 1:5 every 2-5 days, cells may be detached using trypsin/EDTA or mechanically by agitation; seed out at ca. 4 x 106 cells/80 cm2; maintain at 0.3-0.5 x 105 cells/cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-90 hours |
| Harvest: | maximal density of 2 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with ciprofloxacin (Ciprobay), then negative in microbiological culture, RNA hybridization, and continuously in PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | hypertriploid rat karyotype with 20% poylpoidy - 68-71<3n> |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
