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| Cell line: | MOLT-16 |
| DSMZ no.: | ACC 29 |
| Species: | human (Homo sapiens) |
| Cell type: | T cell leukemia |
| Origin: | established from the peripheral blood of a 5-year-old girl with T cell acute lymphoblastic leukemia (T-ALL) at relapse in 1984 (sister cell line MOLT-17, ACC 36) |
| Reference(s): | 14477, 14740 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to elongated single cells un suspension; image; image; image; image; |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/ml; maintain at 0.5-1.5 x 106 cells/ml; split 1:2 to 1:3 twice a week; a significant percentage of the cells may die within the first 24 hours after thawing; we recommend to initiate cell culture in 12- or 24-well plates in the presence of 20% FBS |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | saturation density at 2-2.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD2+, CD3(+), CD4-, CD5+, CD6+, CD7+, CD8(+), CD13-, CD19-, CD34-, TCRalpha/beta(+), TCRgamma/delta-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near diploid karyotype - 46(43-47)<2n>XX, t(3;11)(p21;p13), t(8;14)(q24;q11) - t(8;14)(q24;q11) and 11p13 breakpoint are associated with T-ALL (identical to MOLT-17) |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
