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Cell line: | MOLM-16 |
DSMZ no.: | ACC 555 |
Species: | human (Homo sapiens) |
Cell type: | acute myeloid leukemia |
Origin: | established in 1999 from the peripheral blood of a 77-year-old woman at relapse of acute myeloid leukemia (AML M0) after failed chemotherapy |
Reference(s): | 14747 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | large round cells growing singly or in small clumps in suspension; image; image; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS |
Subculture: | seed out at ca. 0.5 x 106 cells/ml; maintain at ca 0.2-0.8 x x106 cells/ml; do not exceed 1 x 106 cells/ml; split saturated culture 1:2 to 1:4 every 2-5 days |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 50-80 hours |
Harvest: | cell harvest of ca. 1 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD13 +, CD19 -, CD33 +, CD71 +, CD235a +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypotetraploid karyotype with hypodiploid sideline - 83-88<4n>XXX, -X, -4, -4, -5, +6, +7, -9, -18, +2mar, add(5)(q11)x2, add(6)(q11-13), der6)t(6;9(q16;p13), t(6;8)(q15;q24.3), der(8)t(6;8)(q15;q24.3), der(9;18)(p10;q10), der(16)del(16)(p13)add(16)(q11), der(16)(del16)(p13)dup(16) (q?11q?24)ins(16;?)(q11;??), der(18)t(9;18)(q13;q21), der(19)add(19)(p13)add(19)(q13)x2 - resembles published karyotype |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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