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| Cell line: | PAB-100 |
| DSMZ no.: | ACC 188 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | hybridoma (anti-SV40 large T viral antigen) |
| Origin: | established by fusion of NS-1 myeloma cells and spleen cells from a female BALB/c mouse immunized with SVT-2, an SV40-transformed BALB/c mouse cell line; cells reportedly secrete an IgG1 antibody recognizing denaturation-sensitive antigenic determinants unique to large T antigen |
| Reference(s): | 14455, 14583 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single cells growing in suspension; image; image; image |
| Medium: | 80% DMEM + 20% h.i. FBS + non-essential amino acids |
| Subculture: | optimal split ratio 1:2 every 2-3 days; maximum density of about 1.0 x 106 cells/ml should not be exceeded |
| Incubation: | at 37 °C with 5% CO2 |
| Harvest: | cell harvest of about 0.5-1.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | predominantly mouse chromosomal morphology with 3 iso-chromosome markers present in most cells - 140(134-155) |
| Viruses: | ELISA: reverse transcriptase positive |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
