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| Cell line: | MOLP-2 |
| DSMZ no.: | ACC 607 |
| Species: | human (Homo sapiens) |
| Cell type: | multiple myeloma |
| Origin: | established from the peripheral blood of a 55-year-old man with IgD lambda type multiple myeloma in 1991 |
| Reference(s): | 14742 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round to polymorph cells growing singly or in small clumps, ca. 1% giant cells; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml in 24-well plates; split culture 1:2 every 4-6 days; maintain at ca. 0.2-0.8 x 106 cells/ml; after thawing cells may need one week before they start to grow (and they grow very slowly at all) |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 4-7 days |
| Harvest: | maximum density at ca. 1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 +, CD20 -, CD33 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD138 +, sm/cyIgG -, sm/cyIgM -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human highly-rearranged, flat-moded, near-triploid karyotype; 66-74<3n>XX, -Y, +2, -4, +6, +7, +11, -15, +17, -18, -18, +19, +20, -22, -22, -22, +3-5mar, der(X;1)(q10;p10), add(2)(q21), der(2)t(2;15)(q37;q14)x2, der(2)t(1;2)(q2?;p1?6), add(5)(q21), del(5)(q12), add(7)(p21)x2, add(11)(p13), der(16)t(1;16)( p21;q11), add(16)(p11)t(14;16)(q32;q22)t(14;22)(q32;q11.1), der(17)t(17;22)(q25;q11)x2, der(21)t(4;21)(q21.2;p11)inv(4), der(21)t(4;21)(q21.2;p11)inv(4)(?p16?q21)t(4;14)(q?35;q32); sdl with der(6)t(6;18)(q21;q12)x2-3; carries multiple complex rearrangements, including cryptic t(4;14)(p16;q32) rearranging NSD2-FGFR3 (WHSC1-FGFR3; MMSET-FGFR3 with IGH |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
