| | |
|---|
| Cell line: | BL-41 |
| DSMZ no.: | ACC 160 |
| Species: | human (Homo sapiens) |
| Cell type: | Burkitt lymphoma |
| Origin: | established from the tumor tissue of an 8-year-old boy with Burkitt lymphoma; cells were described in the literature to be EBV-negative |
| Reference(s): | 14714, 14434 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, round cells growing non-adherent in suspension; image; image; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS (possibly add 1 mM sodium pyruvate + 50 µM mercaptoethanol) |
| Subculture: | seed out at ca. 0.5 x 106 cells/ml (initially in a 24-well-plate); split saturated culture 1:3 to 1:5 every 2 days; optimal density at about 1.0 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30 hours |
| Harvest: | saturation density at about 2.0 x 106cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | CD3 -, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a (+), CD138 +, HLA-DR +, sm/cyIgG -, sm/cyIgM +, sm/cykappa +, sm/cylambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 7% polyploidy - 48(42-49)<2n>XY, +7, -13, +2mar, add(8)(q24), t(8;14)(q24;q32), der(15)t(13;15)(q13;p11), add(17)(p12), subclonal rearrangements at 1q23, 7p22, 11q13 |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
