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established from the peripheral blood of a 65-year-old man with acute myeloid leukemia (AML FAB M4) in 1986 at diagnosis; cells were described in the literature to express the retinoic acid receptor gene; cell line carries the DNMT3A R635W mutation
80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS
seed out at ca. 0.5-1 x 106 cells/ml; it might be of advantage to start culture initially in a 12- or 24-well-plate; after thawing viability may decrease to about 50% and possibly no cell growth during the first 3-4 days is ibserved (do not dilute); later on maintain at 0.7-1.5 x 106 cells/ml and split growing culture 1:2 to 1:3 once or twice a week
at 37 °C with 5% CO2
about 40-50 hours
maximal density at about 2 x 106 cells/ml
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays