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| Cell line: | NU-DUL-1 |
| DSMZ no.: | ACC 579 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established in 1982 from the cerebrospinal fluid of a 43-year-old man with B-cell non-Hodgkin lymphoma (B-NHL), described at the time as undifferentiated lymphoma; assigned to ABC-like lymphoma subtype (activated B-cell) |
| Reference(s): | 14517, 17354 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | polymorphic cells growing singly in suspension; image |
| Medium: | 85-90% RPMI 1640 + 10-15% h.i. FBS |
| Subculture: | seed out at ca. 0.3 x 106 cells/ml; split saturated culture 1:2 to 1:3 every 3 days; maintain at ca. 0.5-0.8 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-36 hours |
| Harvest: | cell harvest of ca. 1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD20 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD138 -, HLA-DR +, sm/cyIgG +, sm/cyIgM -, sm/cykappa -, sm/cylambda +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 4% polyploidy - 45<2n>X, -Y, der(X)t(X;13)(p11.2;q13), del(3)(q26-28), der(5)t(X;5)(q26;q21), del(6)(p21p22), del(6)(q22), der(8)t(8;14)(q24;q32), add(11)(q23-24), der(14)t(8;14)(q24;q32)t(14;13)(q32;?q14)t(13;X)(?q13;p11.2), der(17)t(17;18)(q22;q21.1), der(18)t(5;18)(q21;q21.1)inv(5)(q21;q35), der(19)t(?17;19)(q22;q13.2) - carries semi-cryptic t(8;14) effecting IGH-MYC rearrangement - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
