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| Cell line: | Maska-98 |
| DSMZ no.: | ACC 956 |
| Species: | human (Homo sapiens) |
| Cell type: | multiple myeloma |
| Origin: | established in 1998 from the bone marrow of a 61-year-old woman with aggressive multiple myeloma; described to produce high level of LDH |
| Reference(s): | 40772 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single round cells growing in suspension, accompanied by a significant amount of cell debris; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at about 0.5-1 x 106 cells/ml; split 1:2 every 2-3 days, maintain at about 0.8 x 106 cells/ml; culture does not reach viabilty higher than 80%; arising debris may mimick a bacterial contamination |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 48 hours |
| Harvest: | max density of 1-1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3-, CD10-, CD13-, CD19-, CD20-, CD34-, CD37-, CD38+, CD80-, CD138+, HLA-DR-, sIgG+, sIgM-, sIg-kappa-, sIg-lambda+, cIgG+, cIgM-, cIg-kappa-, cIg-lambda+, cyCD79a-; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
