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| Cell line: | NALM-19 |
| DSMZ no.: | ACC 522 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell precursor leukemia |
| Origin: | established from the peripheral blood of a 26-year-old man with "acute undifferentiated leukemia" at diagnosis in 1988 |
| Reference(s): | 14741, 14744 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | small single round cells growing in suspension, sometimes in small clumps; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | cells are difficult to culture; seed out at ca. 3 x 106 cells/ml and culture in 24-well-plates all the time; the first 1-3 weeks after thawing cells do not grow or only very slowly (exchange spent medium with dilution); in growth phase, split saturated culture 1:2 every 2-3 days but do not dilute below 2 x 106 cells/ml; maximal density of ca. 4 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50-70 hours |
| Harvest: | cell harvest of ca. 2-4 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD15 +, CD19 +, CD20 -, CD34 -, CD38 +, cyCD79a +, CD80 -, HLA-DR +, sm/cyIgG -, sm/cyIgM -, smkappa -, smlambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy - 47(45-48)<2n>XY,+5, del(9)(p21.2) - revision of published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
