| |
---|
Cell line: | NAMALWA.KN2 |
DSMZ no.: | ACC 68 |
Species: | human (Homo sapiens) |
Cell type: | Burkitt lymphoma |
Origin: | established from the tumor mass of a boy with Burkitt lymphoma; this variant subline from the original NAMALWA (ACC 24) was described in the literature to be characterized by differential expression of immunoglobulin and MYC genes and of some cell surface antigens (see reference); cells were reported to harbor a retrovirus of simian origin, SMRV-H |
Reference(s): | 14584 |
Biosafety level: | 1 |
Risk assessment: | The cell line is positive for EBV (HHV-4) by PCR analysis. However, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were negative by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as latent infection without production of active viruses. A transmission of EBV during handling of the cells is unlikely and thus the cell line is categorized biosafety level 1. Additionally, this cell line carries sequences of the squirrel monkey retrovirus (SMRV). According to a recommendation of the German Central Committee for Biological Safety (ZKBS) the cells have to be handled under biosafety level 2 when the cells are used in genetic engineering projects. |
Permissions and restrictions: | A, C |
| DSMZ Cell Culture Data: |
Morphology: | lymphoblastoid, single cells with irregular forms or small clusters, in suspension; image; image; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | maintain at 0.5-1.5 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Harvest: | maximal density at about 2.0-3.0 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization assays |
Immunology: | CD3 -, CD10 +, CD13 -, CD19 +, CD34 -, CD37 +, CD38 +, cyCD79a +, CD80 +, CD138 -, HLA-DR +, cyIgG -, cyIgM +, cykappa -, cylambda +-; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hypodiploid karyotype with 7% tetraploidy - 45(41-45)<2n>X, -Y - NAMALWA marker chromosomes present |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV +, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV +, SMRV + |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
| Print data sheet |