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Cell line: | NS20Y |
DSMZ no.: | ACC 94 |
Species: | murine (mouse) (Mus musculus) |
Cell type: | neuroblastoma |
Origin: | established from a A/J mouse strain with neuroblastoma C1300; cells were described in the literature to be aneuploid and to contain high specific activity of choline acetyltransferase |
Reference(s): | 14354, 40458 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent cells growing in monolayers or small clumps, round cell body with cellular processes; image; image; image
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Medium: | 90% DMEM + 10% h.i. FBS |
Subculture: | split semi-confluent culture 1:5 to 1:10 using trypsin/EDTA (cells can also be detached by tapping the flask); seed out at ca. 2 x 106 cells/80 cm2; viability might decrease to 50-70%; maximal density at ca. 5-10 x 106 cells/80 cm2 |
Incubation: | at 37 °C with 10% CO2 |
Doubling time: | ca. 30-50 hours |
Harvest: | cell harvest of about 1 x 107 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
Cytogenetics: | murine karyotype |
Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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