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| Cell line: | NU-DHL-1 |
| DSMZ no.: | ACC 583 |
| Species: | human (Homo sapiens) |
| Cell type: | B cell lymphoma |
| Origin: | established from the left inguinal lymph node of a 73-year-old man with B-cell non-Hodgkin lymphoma (B-NHL, diffuse large cell lymphoma, non-cleaved cell type) in 1982; assigned to GCB-like lymphoma subtype (germinal center B-cell). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence
and RNA-Seq) |
| Reference(s): | 14517, 15065, 17354, 17355, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | single, round to polymorph cells growing in suspension; image; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out and maintain at ca. 0.5-1.0 x 106 cells/ml; it is recommended to start culture in a 24-well-plate and with 20% FBS; split saturated culture 1:2 to 1:4 every 2-3 days; viability of culture may drop to about 50% after thawing and may need more than one week to recover |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-50 hours |
| Harvest: | maximum density at about 1 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3-, CD10-, CD13-, CD19+, CD20+, CD34(+), CD37(+), CD38(+), CD80-, CD138-, HLA-DR+,
cyCD79a+, cIgG-, cIgM+, cIg-kappa-, cIg-lambda+, sIgG-, sIgM+, sIg-kappa-, sIg-lambda+; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy - 51(46-53)<2n>XY, +5, +8, +9, +12, +12, t(3;8)(p25;q24), i(5p), dup(7)(q21.3q31.1), der(8)t(3;8)(p25;q24), i(9p), del(12)(q11), i(12p), der(14)t(14;18)(q32;q21)x1-3 - sideline with dup(2)(p2?1p2?4) - carries t(3;8) and t(14;18) effecting respective rearrangements of MYC and IGH-BCL2 - resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
