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Cell line: | OCI-AML3 |
DSMZ no.: | ACC 582 |
Species: | human (Homo sapiens) |
Cell type: | acute myeloid leukemia |
Origin: | established from the peripheral blood of a 57-year-old man with acute myeloid leukemia (AML FAB M4) at diagnosis in 1987; cells carry an NPM1 gene mutation (type A) and the DNMT3A R882C mutation. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
Reference(s): | 15050, 14870, 15051, 16666, 16667, 18187 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single, mostly round cells growing in suspension; image; image |
Medium: | 80-90% alpha-MEM (with ribo- and deoxyribonucleosides) + 10-20% h.i. FBS |
Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml (initially with 20% FBS); split saturated culture 1:3 to 1:4 every 2-3 days; maintain at ca. 0.5-2.0 x 106 cells/ml |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 30-40 hours |
Harvest: | maximum density at about 2.5 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3-, CD4+, CD13+, CD14+, CD15+, CD19-, CD33(+), CD34(+), HLA-DR-, cyCD3-, cyCD68+
; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype - 48(45-50)<2n>X/XY, +1, +5, +8, der(1)t(1;18)(p11;q11), i(5p), del(13)(q13q21), dup(17)(q21q25) - sideline with r(Y)x1-2 - hemizygous for RB1 |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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