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| Cell line: | OCI-M2 |
| DSMZ no.: | ACC 619 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myelocytic leukemia |
| Origin: | established in 1984 from the leukemic cells of a 56-year-old patient with erythroleukemia (AML-M6), representing the end stage of a previously identified myelodysplastic syndrome; described in the literature to differentiate along the erythroid or megakaryocytic/monocytic lineage after stimulation with erythroid inducers or phorbol esters, respectively; described in the literature to constitutively and inducibly secrete platelet-derived growth factor (PDGF)-like proteins; contamination with Mycobacterium was eliminated with kanamycin. Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq) |
| Reference(s): | 14833, 14834, 18187 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly or in clusters in suspension, occasionally a few cells become adherent with elongated morphology; image; image; image; image |
| Medium: | 80% Iscove's MDM + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at 1-1.5 x 106 cells/ml; optimal split ratio 1:3 to 1:5 every 3-4 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 36 hours |
| Harvest: | maximum density at ca. 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD14 -, CD19 -, CD33 (+), CD71 +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hyperdiploid karyotype with 4% polyploidy; 51(46-51)<2n>XX, +6, +8, +20, -21, +3mar, der(X)t(X;8)(q23;q23), add(9)(p23)del(9)(p12p21), t(10;12)(p12;p12), del(17)(q11q21.1); no reference karyotype available |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
