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| Cell line: | RH-18 |
| DSMZ no.: | ACC 491 |
| Species: | human (Homo sapiens) |
| Cell type: | rhabdomyosarcoma |
| Origin: | cell line was derived from the tumor material xenografted in immuno-deficient mice; tumor material: primary pediatric embryonal rhabdomyosarcoma exhibiting moderate histological differentiation |
| Reference(s): | 14603, 14467, 18250, 18251, 40521 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | The cell line will only be distributed to non-profit institutions |
| | DSMZ Cell Culture Data: |
| Morphology: | large transparent epitheloid cells, partially with long extensions, growing as monolayers; image; image; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1 x 106 cells/75 cm2; cells grow rather slowly after thawing; later, split culture 1:3 every 3-6 days (before reaching confluency) using trypsin/EDTA; confluency of the culture might result in reduced growth rate after passaging. |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 60-70 hours |
| Harvest: | maximum cell harvest of ca. 4 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat moded hypotetraploid karyotype with 20% polyploidy - 78-87<4n>XX, -3, -4, -4, +5, +7, +7, -10, -10, -12, -12, -15, -19, +20, +20, +20, -21, +1-2mar, +0-4 dmin, add(1)(p11), del(5)(q13), add(16)(p13), del(16)(p13.2) - sideline with i(3q) |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
