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| Cell line: | RPMI-2650 |
| DSMZ no.: | ACC 287 |
| Species: | human (Homo sapiens) |
| Cell type: | nasal septum squamous cell carinoma |
| Origin: | from the pleural effusion of a 52-year-old man with anaplastic squamous cell carcinoma of the nasal septum in 1962 |
| Reference(s): | 14780, 14781 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, epitheloid, very small cells growing in clusters; image; image |
| Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS + 1x non-essential amino acids |
| Subculture: | split confluent culture 1:4 to 1:8 every 4-5 days using trypsin/EDTA; seed out at about 0.5-1.0 x 106 cells/25 cm2; viability may decrease directly after thawing but cells do recover rapidly |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 40-50 hours |
| Harvest: | cell harvest of about 0.6 x 106 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human near-diploid karyotype with 10% polyploidy - 45(43-49)<2n>XY, -4, -16, -18, +2mar, del(1)(p11), add(4)(p16), del(8)(q22), add(14)(p11), add(14)(q32), del(19)(p13) - resembles published karyotype |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
