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| Cell line: | SER-W3 |
| DSMZ no.: | ACC 420 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | Sertoli cells |
| Origin: | Sertoli cells dissected from normal testis from 17-day-old Wistar rat and immortalized with SV40 large-T antigen; cells were described as displaying morphological and functional features of Sertoli cells (phagocytosis, cisplatin sensitivity, transferrin secretion) |
| Reference(s): | 14855 |
| Biosafety level: | 1, GMO-S1 |
| Risk assessment: | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epitheloid cells growing in monolayers; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture: | split 90% confluent culture 1:2 to 1:5 every 2-6 days using trypsin/EDTA; seed out at ca. 0.3-0.5 x 105 cells/cm2; maintain at ca. 0.1-0.3 x 105 cells/cm2 |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 40-50 hours |
| Harvest: | cell harvest of ca. 0.8 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | rat flat-moded hypotetraploid karyotype - 70-78<4n>XXYY/XXY - supernumerary r/acf present in most cells |
| Viruses: | ELISA: reverse transcriptase negative; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
