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Cell line: | SF-158 |
DSMZ no.: | ACC 292 |
Species: | insect - fall armyworm (Spodoptera frugiperda) (Spodoptera frugiperda) |
Cell type: | |
Origin: | cloned from the parent line, IPLB-SF-21-AE, derived from pupal ovarian tissue of the fall armyworm (species Spodoptera frugiperda, family Noctuidae, order Lepidoptera); cells are susceptible to infection with MNPV and other Baculoviruses; cells can be used with Baculovirus expression vectors |
Reference(s): | 14532 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | polymorphic round cells, mostly adherent, a few cells in suspension; image; image; image |
Medium: | 80-90% TC 100 medium + 10-20% h.i. FBS (or 80-90% Grace´s insect medium + 10-20% h.i. FBS supplemented with L-Glutamine, hydrolyzed lactalbumin and yeastolate) |
Subculture: | split culture 1:4 to 1:5 every 5-6 day (cells grow adherent, but trypsin is not necessary as cells can be detached by tapping the flask or with a rubber scraper); seed out about 1-2 x 106 cells/25 cm2 |
Incubation: | 25-29 °C without CO2 ; temperature below 23 °C may lead to reduced growth and loss of adherence |
Doubling time: | about 70 hours (at room temperature) |
Harvest: | cell harvest of about 0.2 x 106 cells/cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Spodoptera frugiperda species. |
Viruses: | PCR: SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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