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| Cell line: | TLBR-3 |
| DSMZ no.: | ACC 906 |
| Species: | human (Homo sapiens) |
| Cell type: | anaplastic large cell lymphoma |
| Origin: | established in 2011 from primary tumor tissue of a breast implant-associated T-cell anaplastic large cell lymphoma of a 45-year-old-woman; cells are IL-2 dependent and are reported to secrete several cytokines. ALK-negative and up-regulation of survivin, BCL2, NOTCH-1 and NOTCH-1 receptors; sensitive to doxorubicin and honokiol (a SHP-1 activator) |
| Reference(s): | 40178, 40179, 40183 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly and in small aggregates in suspension; image; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS + 10ng/ml IL-2 |
| Subculture: | seed out at about 0.5 x 106 cells/ml; maintain at 0.2-1 x 106 cells/ml; split 1:3 to 1:8 every 2-4 days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | about 20-50 hours |
| Harvest: | maximal density of ca. 1.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD4 +, CD5 -, CD8 -, CD10 -, CD20 -, CD21 -, CD25 +, CD30 +, CD71 +; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | confirmed triploidy; resembles published karyotype with varying gains and losses of single chromosomes |
| Molec. Genetics: | absence of fusion gene NPM1-ALK confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
