| | |
|---|
| Cell line: | U-2-OS |
| DSMZ no.: | ACC 785 |
| Species: | human (Homo sapiens) |
| Cell type: | osteosarcoma |
| Origin: | established in 1964 from a moderately differentiated osteogenic sarcoma of the tibia of a 15-year-old girl; cells were described in the literature to secrete osteosarcoma-derived growth factor (ODGF), insulin-like growth factor (IGF-1) receptor, insulin-like growth factor-II/Mannose-6-phosphate (IGF-II/M6P) receptor, and IGF-II; the cells were originally designated 2T |
| Reference(s): | 24159, 24160, 24161, 24162 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently as monolayer; image; image; image; image; image; image |
| Medium: | 90% McCoy's 5A + 10% h.i. FBS |
| Subculture: | seed out at ca. 1-2 x 106 cells/175 cm2 flask; split confluent culture 1:10 - 1:20 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 25-30 hours |
| Harvest: | cell harvest of ca. 15 x 106 cells/175 cm2 |
| Storage: | frozen in serum-free freezing medium (BambankerTM) |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
