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| Cell line: | U2OS#18-RAD51C-15 |
| DSMZ no.: | ACC 834 |
| Species: | human (Homo sapiens) |
| Cell type: | osteosarcoma |
| Origin: | Genetic modification of U2OS#18 (DSMZ ACC 832, a reporter cell line for measurement of sister chromatid recombination, SCR) with a genetic disruption of RAD51C by frameshifting mutations in exon 1 (targeted by
CRISPR-CAS9; puromycin resistant and expressing GFP). The wild-type allele is no longer present. A sister cell line with the retrovirally reconstituted RAD51C gene is available as ACC 839 |
| Reference(s): | 18169, 18205, 18186 |
| Biosafety level: | 1, GMO-S1 |
| Risk assessment: | genetically modified organism, according to German and European legislation allocated into group S1 |
| Permissions and restrictions: | A, D |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelioid cells growing adherently as monolayer; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS + (additionally) 2 mM L-glutamine (to verify puromycin resistance, 1 µg/ml should be added) |
| Subculture: | seed out at 0.5-1 x 106 cells/80 cm2;
split confluent culture once or twice a week 1:3 to 1:4 using trypsin/EDTA |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 50-60 hours |
| Harvest: | cell harvest of 8-10 x 106 cells/175 cm2 flask |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | to inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Molec. Genetics: | frameshift mutations in exon 1 of RAD51C were verified by sequence analysis |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
