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established from the pericardial fluid of a 15-year-old man with acute lymphoblastic leukemia (ALL) at relapse (with accompanying thymoma) in 1983; cells were described as: (i) showing NK activity and ADCC (antigen-dependent cell-mediated cytotoxicity), (ii) having their T cell receptor (TCR) genes in germline configuration, and (iii) expressing no TCR proteins (also CD3-, CD16-, CD56+, CD57-). Exome and RNA sequence data are available (see Ref 18187 and
Exome sequence and RNA-Seq)
The cell line is positive for EBV (HHV-4) by PCR analysis. Additionally, expression of immediate-early protein BZLF-1 and lately expressed capsid protein were also positive by western blot and immunostaining, respectively, in untreated and phorbol ester / sodium butyrate stimulated cells. The infection was classified as lytic associated with a production of active viruses. A transmission of EBV during handling of the cells is possible and the cell line is thus categorized biosafety level 2.
round to polygonal cells growing singly or in clumps in suspension; image; image; image
80% Iscove's MDM + 20% h.i. FBS
start culture in a 24-well-plate and seed out at ca. 0.8-1.5 x 106 cells/ml, initially after thawing the viability may be reduced down to ca. 20-50% during the first 1-2 weeks (during that initial period, cells should not be diluted, medium may be exchanged by centrifugation); in their vigorous growth phase, maintain at 0.2-0.5 x 106 cells/ml and split saturated culture 1:2 every second day; a significant amount of cell debris may allways be present in the culture
at 37 °C with 5% CO2
ca. 40-50 hours
cell harvest of ca. 0.6 x 106 cells/ml
frozen with 70% medium, 20% FBS, 10% DMSO
DSMZ Scientific Data:
negative in DAPI, microbiological culture, RNA hybridization, PCR assays