| | |
|---|
| Cell line: | FLK-BLV-044 |
| DSMZ no.: | ACC 153 |
| Species: | ovine (sheep) (Ovis aries) |
| Cell type: | embryonal kidney cells |
| Origin: | established from the embryonal kidney cells of an ovine in 1976; cells are susceptible to infection with BLV (bovine leukemia virus) leading to the formation of syncythia |
| Reference(s): | not published |
| Biosafety level: | 1 |
| Risk assessment: | The cell line is infected with bovine leukemia virus (BLV) and produces active viruses. The viruses are not pathogenic to humans, but may be pathogenic to animals. |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | large fibroblast-like cells growing as monolayers; image; image; image; image; image; image |
| Medium: | 90% MEM (with Earle's salts) + 10% h.i. FBS optional: + 850 mg/L NaHCO3 + 120 mg/L sodium pyruvate + 1x non-essential amino acids |
| Subculture: | seed out about 1-2 x 106 cells/80 cm2;
split confluent culture 1:2 to 1:8 every 3-5 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 50 hours |
| Harvest: | cell harvest of about 4 x 106/80 cm2 |
| Storage: | frozen with 82.5% medium, 10% FBS, 7.5% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Ovis aries species. |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
