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Cell line: | IPL-LD-65Y |
DSMZ no.: | ACC 181 |
Species: | insect - gypsy moth (Lymantria dispar) (Lymantria dispar) |
Cell type: | larval cells |
Origin: | established from the larvae of a gypsy moth (species Lymantria dispar, family Lymantriidae, order Lepidoptera) (Schwammspinner) |
Reference(s): | not published |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | large cells; up to 30% grow adherent with processes; suspension cells are round to oval; image; image |
Medium: | 80% TC 100 medium + 20% h.i. FBS |
Subculture: | cells are difficult to culture and grow slowly! split confluent culture 1:4 every 4-5 days using a (plastic) cell scraper |
Incubation: | without CO2 at room temperature (25-28 °C) |
Harvest: | cell harvest of up to 10 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Lymantria dispar species. |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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