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Cell line: | IGR-39 |
DSMZ no.: | ACC 239 |
Species: | human (Homo sapiens) |
Cell type: | melanoma |
Origin: | established from the primary (achromic) cutaneous tumor (left thigh) of a 26-year-old man with malignant melanoma (primary tumor histology: SSM level IV); a cell line IGR-39 from a lymph node metastasis is available (DSMZ ACC 237); described as tumorigenic in nude mice |
Reference(s): | 14363, 40558 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | adherent epithelial-like cells growing in monolayers; image; image |
Medium: | 85% DMEM + 15% h.i. FBS |
Subculture: | seed out at ca. 1 x 106 cells/80 cm2; split subconfluent or confluent culture 1:4 every 3-4 days using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | about 30 hours |
Harvest: | maximal density of about 6-8 x 106/175 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | contamination was eliminated with BM-Cyclin (tiamulin & minocycline), then negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database matching the profile of sister cell line IGR-37 |
Cytogenetics: | human flat-moded tetra-/pentaploid karyotype - 104-112<4n>XXY - rearrangements include der(1)t(1;?21)(p13;q11), der(16)t(1;16)(p13;q23) also present in the autologous sister line IGR-37 |
Molec. Genetics: | Genomic sequencing and/or ARMS assay show that the cell line carries the BRAF V600E mutation (mu/wt), but no NRAS Q61 mutation |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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