| | |
|---|
| Cell line: | BETA-TC-3 |
| DSMZ no.: | ACC 324 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | pancreas insulinoma (beta-tumor) |
| Origin: | established from pancreatic insulinoma excised from a transgenic mouse carrying hybrid insulin-SV40 large T genes in 1988; described as producing pro-insulin I and II processed into mature insulin (inducible 30-fold by glucose); however, the DSMZ did not verify the production of insulin |
| Reference(s): | 14957, 14501 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent cells growing as multilayers; image; image; image; image; image; image; image |
| Medium: | 82.5% DMEM (4.5 g/L glucose) + 15% horse serum + 2.5% h.i. FBS |
| Subculture: | seed out at ca. 1-4 x 106 cells/175 cm2; split confluent culture 1:5 to 1:10 every 2-5 days by mechanical disruption (trypsin/EDTA can be used for a very short time); the DSMZ cannot guarantee the production of insulin |
| Incubation: | at 37 °C with 7.5-10% CO2 |
| Doubling time: | ca. 34 hours |
| Harvest: | cell harvest of ca. 2-3 x 105 cells/cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hypotetraploid karyotype - 74(70-78)<4n> - 2-4 centric fusion markers and 1-3 dmin present |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
