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Cell line: | DBTRG-05MG |
DSMZ no.: | ACC 359 |
Species: | human (Homo sapiens) |
Cell type: | glioblastoma |
Origin: | established from the primary tumor cells of a 59-year-old woman with glioblastoma multiforme following irradiation and chemotherapy in 1985; cells were described in the literature to express NSE (neuron-specific enolase) and EGF-R (epidermal growth factor receptor) |
Reference(s): | 14688 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | spindle, fibroblast-like cells growing in monolayers; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 2 x 106 cells/80 cm2; split confluent culture 1:3 to 1:5 twice a week using trypsin/EDTA |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 48 hours |
Harvest: | cell harvest of ca. 10 x 106 cells/80 cm2 |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | initial contamination was eliminated with Mycoplasma Removal Agent, then negative in DAPI, microbiological culture, RNA hybridization, and continuously in PCR assays |
Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human flat-moded hypotetraploid karyotype with 12% polyploidy - 81-88<4n>XXX, -X, -1, -1, -3, -4, -4, +7, +7, -10, -10, -13, -13, -14, -14, -16, +6mar, der(4)t(4;14)(p11;q11), add(5)(p15)x2, add(7)(p22)x3, del(8)(q22), inv(9)(p13q34)x1-2, add(11)(p15)x2, der(11)t(1;11)(q23;q21), del(11)(q13), add(16)(q24)x2 - +7 and -10 recurrent in gliomas - matches published karyotype |
Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HHV-8 -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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