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| Cell line: | SCLC-22H |
| DSMZ no.: | ACC 373 |
| Species: | human (Homo sapiens) |
| Cell type: | small cell lung carcinoma |
| Origin: | established from the pericardial effusion of a 46-year-old man with small cell lung carcinoma, oat cell type; patient had received chemotherapy; sister cell line of SCLC-21H (DSMZ ACC 372); listed in the CCLE (ref 18232) |
| Reference(s): | 14381, 14382, 14383, 18232 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | growing in small clumps and spheroids in suspension; image; image; image |
| Medium: | 90% DMEM + 10% h.i. FBS |
| Subculture: | seed out all cells in a T75 flask with ca. 20ml medium; take care not to disrupt the cells
(use pipettes with wide opening like serological, wide-bore or cut) avoid standard pipettes, work slowly and carefully |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 48 hours |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human hypodiploid karyotype with 8% polyploidy - 43(40-44)<2n>XY, -8, -10, +11, +12, -16, -17, del(4)(q32), add(5)(p15), del(11)(p11), t(12;17)(q10;q10), add(12)(q23), del(13)(q12q14), add(21)(p11), add(22)(q13) - sideline with del(3)(p14) - closely resembles karyotype of sister cell line SCLC-21H |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
