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| Cell line: | BHY |
| DSMZ no.: | ACC 404 |
| Species: | human (Homo sapiens) |
| Cell type: | oral squamous cell carcinoma |
| Origin: | established from the tumor of a 52-year-old man with highly differentiated squamous cell carcinoma of the lower alveolus which was highly invasive to the mandibular bone and the muscle layer of the oral floor; described as anchorage-independent and to be tumorigenic in nude mice |
| Reference(s): | 14652 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial-like polygonal or round and flat cells (sometimes spindle-form, very heterogenous) growing in monolayers; image; image; image; image |
| Medium: | 90% DMEM (4.5 g/L gluocse) + 10% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells in a T75 flask in 15 ml medium; split confluent culture 1:2 to 1:3 every 3-5 days using trypsin/EDTA |
| Incubation: | at 37 °C with 10% CO2 |
| Doubling time: | ca. 70 hours |
| Harvest: | cell harvest of ca. 7 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | initial contamination was eliminated with Baytril (enrofloxacin), then negative in DAPI, microbiological culture, RNA hybridization and continuously in PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded hypotriploid karyotype with 28% polyploidy - 61-68<3n>XX, -Y, -1, -2, +4, -5, -6, +9, +11, -13, +15, -17, -18, +20, +20, -21, +3-4mar, del(X)(q11q21), add(1)(q11), ?inv(2)(p17q11), add(2)(q11), del(3)(p11p14.3)x2, i(4p), add(4)(q3?1), del(5)(q13), add(5)(p15)x1-2, der(8;10)(q10;q10), add(9)(q34), add(9)(p11), del(10)(p13), del(10)(q23), der(11)t(1;11)(q11;q25), add(12)(p11), add(12)(p11), add(12)(q13-21), add(13)(p11), add(16)(q24), del(16)(p13), add(17)(p12), add(18)(p11), add(19)(q13) - 3-10 dmin counted in some cells |
| Viruses: | ELISA: reverse transcriptase negative; PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-1/2 -, MLV+, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
