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| Cell line: | RLD-1 |
| DSMZ no.: | ACC 415 |
| Species: | murine (mouse) (Mus musculus) |
| Cell type: | T cell leukemia |
| Origin: | established from transplantable tumors induced in syngeneic mice inoculated with cell suspensions prepared from lymph nodes infiltrated by lymphatic leukemia induced in 6-week-old DBA/2 mice by Rauscher virus (present in supernate of JLSV-9 cells); described as expressing T cell surface markers (Thy-1, T-200, Lyt-1), high constitutive TdT activity, and as differentiating along T-helper lineage in response to treatment with DMSO or phorbol ester TPA |
| Reference(s): | 14451, 14589 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | spheroidal cells growing singly in suspension; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS |
| Subculture: | seed out at ca. 0.3-0.5 x 106 cells/ml; maintain at 0.2-0.7 x 106 cells/ml; optimal split ratio of 1:2 to 1:6 every 2-4 days; cell growth may be slow for the first few days |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 20-30 hours |
| Harvest: | saturation density of ca. 1.5-2.0 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in DAPI, microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Mus musculus species. |
| Cytogenetics: | murine hyperdiploid karyotype - 42<2n>XX, +14, +15 - no centric fusion markers present - matches published karyotype |
| Viruses: | ELISA: reverse transcriptase positive; PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
