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| Cell line: | HH-16 cl.2/1 |
| DSMZ no.: | ACC 485 |
| Species: | rat (Rattus norvegicus) |
| Cell type: | mammary fibrosarcoma |
| Origin: | established from the ascitic fluid of a rat with a tumor which was produced by injecting cultured cells of a chemically-induced (dimethylbenzanthracene) mammary tumor; rat: strain Sprague-Dawley, female, 240 days old, adult; the original tumor was induced by single feeding of DMBA on day 45 after birth; sister cell line of HH-16 cl. 4 (DSM ACC 358); cells were described in the literature to produce invasive fibrocarcinomas in S.D. rats upon s.c. injection with remote metastases |
| Reference(s): | 14925, 14981, 14617, 14982 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | fibroblastoid growing in a criss-cross pattern; image; image; image |
| Medium: | 90% RPMI 1640 + 10% h.i. FBS + 2 mM L-glutamine |
| Subculture: | split confluent culture 1:4 every 3-4 days using trypsin/EDTA (NB: cells are sensitive to trypsin/EDTA and should not be incubated for more than 5 min at room temperature); seed out at ca. 1-2 x 106 cells/80 cm2; maintain at 4-5 x 106 cells/80 cm2 |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40 hours |
| Harvest: | cell harvest of ca. 7-8 x 106 cells/80 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, RNA hybridization, PCR assays |
| Species validation: | Species-level identification was carried out by mitochondrial Cytochrome C Oxidase Subunit 1 (COI) DNA barcoding according standard ANSI/ATCC ASN-0003-2015 and revealed Rattus norvegicus species. |
| Cytogenetics: | rat near diploid karyotype with 11% polyploidy - 39-43<2n> |
| Viruses: | PCR: SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
