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Cell line: | NOMO-1 |
DSMZ no.: | ACC 542 |
Species: | human (Homo sapiens) |
Cell type: | acute myeloid leukemia |
Origin: | established from the bone marrow of a 31-year-old woman with acute myeloid leukemia (AML FAB M5a) at 2nd relapse; described in the literature to show strong lysozyme activity in the cytoplasm, phagocytic activities, and cells are responsive to induction of differentiation with TPA; cells carry the t(9;11)(p22;q23) KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) alteration |
Reference(s): | 14595, 15031, 14493, 15144, 16555 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
| DSMZ Cell Culture Data: |
Morphology: | single round cells growing in suspension; image; image; image |
Medium: | 90% RPMI 1640 + 10% h.i. FBS |
Subculture: | seed out at ca. 0.5-1.0 x 106 cells/ml; after thawing cell growth may be retarded; maintain at ca. 0.5 x 106 cells/ml; split saturated culture 1:2 to 1:4 twice a week |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 35-40 hours |
Harvest: | cell harvest of ca. 1.5-2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in microbiological culture, PCR assays |
Immunology: | CD3 -, CD4 +, CD13 +, CD14 -, CD15 +, CD19 -, CD33 +, CD34 -, HLA-DR +; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Cytogenetics: | human hyperdiploid karyotype with 8% polyploidy - 46-47<2n>XX, +8, -13, +mar, add(7)(q32), der(9)del(9)(p11p13-21)t(9;11)(p22;q23), t(9;13)(q13;q11), der(11)t(9;11)(p22;q23) - carries reciprocal t(9;11) with rearrangement of KMT2A (MLL) - matches published karyotype |
Molec. Genetics: | expression of fusion gene KMT2A-MLLT3 (MLL-MLLT3; MLL-AF9) confirmed by RT-PCR |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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