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| Cell line: | JL-1 |
| DSMZ no.: | ACC 596 |
| Species: | human (Homo sapiens) |
| Cell type: | epithelioid mesothelioma |
| Origin: | established from the untreated malignant epithelioid mesothelioma of the pleura of a 54-year-old man with asbestos cancer in 2003 |
| Reference(s): | 14850 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | adherent, epithelial-like cells growing in monolayers; image; image; image |
| Medium: | 80% DMEM or NCTC-109 + 20% h.i. FBS + 2 mM L-glutamine |
| Subculture: | seed out at ca.1- 2 x 106 cells/175 cm2; split confluent culture 1:5 once or twice a week using trypsin/EDTA |
| Incubation: | at 37 °C with 10% CO2 (Dulbecco´s MEM) or 5% CO2 (NCTC-109) |
| Doubling time: | ca. 70-80 hours |
| Harvest: | cell harvest of ca. 5-7 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human flat-moded karyotype with 12 % polyploidy; 42-47<2n>XY, +5, +7, -10, -13, +20, der(1)t(1;15)(q11;q11), inv(3)(p1?q2?), der(5)t(5;16)(q11;?), add(10)(q25), der(11)t(10;11)(q21;q23), der(14)t(1;14)(q11;q31); sdl with add(9)(p11), del(16)(q11); karyotype displays significant intercellular differences;
resembles published karyotype |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
