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| Cell line: | SHI-1 |
| DSMZ no.: | ACC 645 |
| Species: | human (Homo sapiens) |
| Cell type: | acute myelocytic leukemia |
| Origin: | established in 2002 from the bone marrow of a 37-year-old male with relapsed acute monocytic leukemia (AML-M5b); described to carry a TP53 point mutation; carries a t(6;11)(q27;q23) and corresponding KMT2A-AFDN (MLL-MLLT4; MLL-AF6) fusion gene |
| Reference(s): | 14429 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | round cells growing singly in suspension; image; image; image; image |
| Medium: | 80-90% Iscove's MDM + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 0.5-1.0 x 106 cells/ml; optimal split ratio of 1:4 to 1:10 every 2-4 days; maintain at 0.3-1.5 x 106 cells/ml |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 40-60 hours |
| Harvest: | maximum densitiy of ca. 2.5 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | CD3 -, CD13 +, CD15 +, CD19 -, CD33 +, CD34 -, CD235a -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Cytogenetics: | human pseudodiploid karyotype with 12% polyploidy; 46(44-46)<2n>XY, -18, +mar, t(6;11)(q27;q23), add(8)(p23), del(10)(p11), del(17)(p12); carries t(6;11) effecting rearrangement of KMT2A (MLL) with AFDN (MLLT4; AF6); matches published karyotype |
| Molec. Genetics: | expression of fusion gene KMT2A-AFDN (MLL-MLLT4; MLL-AF6) confirmed by RT-PCR |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-1/2 -, MLV -, SMRV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
