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| Cell line: | SK-GT-4 |
| DSMZ no.: | ACC 712 |
| Species: | human (Homo sapiens) |
| Cell type: | esophageal adenocarcinoma |
| Origin: | established in 1989 from the primary tumor of an 89-year-old man with an adenocarcinoma of the distal esophagus; described to carry TP53, KRAS,and PIK3CA mutations; whole genome sequence data in ref 40877 |
| Reference(s): | 16055, 16343, 16403, 40877 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| | DSMZ Cell Culture Data: |
| Morphology: | epithelial cells growing adherently as monolayer; image
; image |
| Medium: | 80-90% RPMI 1640 + 10-20% h.i. FBS |
| Subculture: | seed out at ca. 1 x 106 cells/175 cm2 flask; split culture 1:5 to 1:10 every 4-6 days using trypsin/EDTA |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 30-35 hours |
| Harvest: | cell harvest of ca. 20 x 106 cells/175 cm2 |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in microbiological culture, PCR assays |
| Immunology: | To inquire about expression of EpCAM and intermediate filaments, contact ulfert.rand@dsmz.de. |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HPV -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | | Frozen culture | | 450,- € | Growing culture
(please inquire for exact delivery time) | | 900,- € | | DNA isolated from cell line (25 µg) | | 560,- € | | DNA isolated from cell line (5 µg) | | 140,- € |
see price list |
| | Print data sheet |
