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Cell line: | LOPRA-1 |
DSMZ no.: | ACC 778 |
Species: | human (Homo sapiens) |
Cell type: | multiple myeloma |
Origin: | established in 1982 from ascites fluid containing malignant plasma cells of a 62-year-old woman with fatal multiple myeloma (stage IIA, IgA2/kappa) after chemotherapy; described in the literature to be growth-dependent on IL-6 |
Reference(s): | 18122 |
Biosafety level: | 1 |
Permissions and restrictions: | A |
Depositor-specific restrictions: | Transfer to third parties needs to be negotiated with the depositor. |
| DSMZ Cell Culture Data: |
Morphology: | variously sized round to orthogonal cells growing in suspension and occasionally in small clumps; image; image |
Medium: | 80% RPMI 1640 + 20% h.i. FBS + 10 ng/ml human IL-6 |
Subculture: | seed out at ca. 1 x 106 cells/well in 24-well-plates; split saturated culture 1:2 every 4-6 days; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
Incubation: | at 37 °C with 5% CO2 |
Doubling time: | ca. 5 days |
Harvest: | maximum density at ca. 2 x 106 cells/ml |
Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| DSMZ Scientific Data: |
Mycoplasma: | negative in PCR assay |
Immunology: | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD28 +, CD34 -, CD37 -, CD38 +, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
Supplied as: | Delivery form | | Prices | | Frozen culture | | 450,- € | Growing culture (please inquire for exact delivery time) | | 900,- € | DNA isolated from cell line (25 µg) | | 560,- € | DNA isolated from cell line (5 µg) | | 140,- € | see price list |
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