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| Cell line: | LOPRA-1 |
| DSMZ no.: | ACC 778 |
| Species: | human (Homo sapiens) |
| Cell type: | multiple myeloma |
| Origin: | established in 1982 from ascites fluid containing malignant plasma cells of a 62-year-old woman with fatal multiple myeloma (stage IIA, IgA2/kappa) after chemotherapy; described in the literature to be growth-dependent on IL-6 |
| Reference(s): | 18122 |
| Biosafety level: | 1 |
| Permissions and restrictions: | A |
| | DSMZ Cell Culture Data: |
| Morphology: | variously sized round to orthogonal cells growing in suspension and occasionally in small clumps; image; image |
| Medium: | 80% RPMI 1640 + 20% h.i. FBS + 10 ng/ml human IL-6 |
| Subculture: | seed out at ca. 1 x 106 cells/well in 24-well-plates; split saturated culture 1:2 every 4-6 days; CAVEAT: by favoring selective growth, suboptimal culture of cytokine-dependent cell lines may promote outgrowth of factor-independent subclones. Selective conditions include cytokine insufficiency and inadequate cell density. Thus the DSMZ cannot guarantee indefinite stability of factor-dependence of cell lines. |
| Incubation: | at 37 °C with 5% CO2 |
| Doubling time: | ca. 5 days |
| Harvest: | maximum density at ca. 2 x 106 cells/ml |
| Storage: | frozen with 70% medium, 20% FBS, 10% DMSO |
| | DSMZ Scientific Data: |
| Mycoplasma: | negative in PCR assay |
| Immunology: | CD3 -, CD10 -, CD13 -, CD19 -, CD20 -, CD28 +, CD34 -, CD37 -, CD38 +, CD80 -, CD138 +, HLA-DR -, sm/cyIgG -, sm/cyIgM -, sm/cykappa +, sm/cylambda -; image |
| Authenticity: | STR analysis according to the global standard ANSI/ATCC ASN-0002.1-2021 (2021) resulted in an authentic STR profile of the reference STR database. |
| Viruses: | PCR: EBV -, HBV -, HCV -, HIV-1 -, HIV-2 -, HTLV-I/-II -, MLV - |
| Supplied as: | | Delivery form | | Prices | | Growing culture
(please inquire for exact delivery time) | | 1040,- € | | Frozen culture | | 520,- € | | DNA isolated from cell line (25 µg) | | 650,- € | | DNA isolated from cell line (5 µg) | | 160,- € |
see price list |
| | Print data sheet |
